功能小分子平台

平台负责人

朱晓玥教授(hbmc_fcgc@163.com)

地址

福建省福州市福建农林大学海峡联合研究院609室  邮编：350002

平台简介

植物化学基因组学平台面向全国高等院校、科研机构提供高通量化合物筛选公共技术服务。平台目前拥有植物天然小分子化合物库以及Tecan Freedom EVO^ 200全自动化液体处理工作站、Spark^多功能微孔板检测仪、Cytomat™全自动二氧化碳培养箱等全球领先设备，可实现高通量化合物筛选、酶联免疫吸附实验、筛选模型建立等过程的全自动化操作。平台也配备了步入式恒温恒湿洁净室和细胞培养所需关键仪器设备，并提供相应技术支撑服务，满足开展高水平化学功能基因组学研究的创新需求。

设备清单和主要技术指标

Tecan Freedom EVO® 200大型全自动化液体处理工作站

灵活、成熟的多功能机器人平台，为多样本和高通量应用提供自动化解决方案。包括以下功能模块：

• MCA 96通道移液机械臂、灵活8通道移液机械臂LiHa和移板机械臂RoMa，可以多个机械臂并行高速高通量处理。

 MCA96通道移液机械臂

加样精度：1𝜇l：CV<6%；5𝜇l：CV<4%；>10𝜇l：CV<3%

分配96孔样品或试剂（50𝜇l/孔）≤ 30秒/板

• 灵活8通道移液机械臂LiHa

可使用多种类型的吸头

 8个加样器Y轴间距可变（范围9-38mm），Z轴独立

每根针均具备独立的电容式液面感应功能

加样精度：10𝜇l CV<2.5%；100𝜇l CV<0.5%

• 独立的移板机械臂RoMa

任意两点之间位移小于10秒

具有平板抓手适配器可以抓取90mm直径平板

• 振荡器

频率可调（100-1500rpm）

圆形振荡

 2mm振幅

震荡时间可调

• 控温孵育塔

 6微孔板位，密闭式孵育，避光

循环热气浴式加热

温控范围：室温+5℃至46℃，控温精确度：±0.5℃

• Hydrospeed™ 高速洗板机

整板清洗，兼容96孔/384孔微孔板

支持ELISA清洗，细胞超柔清洗

96孔板清洗时间 1个清洗循环（包括两点吸液步骤）15秒，（注液量：300𝜇l/孔；注液速率5；吸液速率5；96HT 清洗头）

384孔板清洗时间 1个清洗循环（包括两点吸液步骤）15秒，（注液量：100𝜇l/孔；注液速率5；吸液速率5；384HT 清洗头）

注液准确度@ 300𝜇l，96孔板 ≤5% （称重法）

注液准确度@ 100𝜇l，384孔板 ≤5% （称重法）

注液均一度@ 300𝜇l，96孔板 ≤3%整板

注液均一度@ 100𝜇l，384孔板 ≤4%整板

残留量，96孔板 < 2𝜇l/孔 2点吸液

残留量，384孔板 < 2𝜇l/孔 单点吸液

真空度范围 -50～-300mBar

• Spark®多功能微孔板检测仪

光吸收

荧光强度

时间分辨荧光

荧光偏振

化学发光

• Cytomat™全自动二氧化碳培养箱

自动存取任意位置的微孔板，存取时间为20秒/块

容量：42块96孔/384孔标准板或20块96孔深孔板或56块1536孔板

温控范围：室温+8℃~50℃

温控精度：±0.1℃

最大相对湿度：95%

 CO₂浓度控制范围：0~20%

• 高效空气过滤模块

苏州净化百级HEPA层流罩，为工作站提供洁净级的空气，避免细胞培养中的细菌/真菌感染

步入式恒温恒湿洁净室及动物细胞培养设备

• 十万级步入式恒温恒湿洁净室

• Thermofisher II级/A2型生物安全柜

• Thermofisher 4100水套式CO₂培养箱

• Thermofisher STERI-CYCLEi250三气培养箱

• Millipore Scepter 2.0 手持式细胞计数器

• Nikon Ts2-FL倒置荧光显微镜

• TokaiHit TPi-TS2X 显微恒温加热系统

• Thermofisher Locator JR液氮存储器

• 天平

• 离心机

• 恒温振荡水槽

Functional Chemical Genomics Core

Manager

Xiaoyue Zhu, Ph.D. (hbmc_fcgc@163.com)

Core Location

Haixia Institute for Science and Technology Room 609

Fujian Agriculture and Forestry University

15 Shangxiadian Road

Fuzhou, Fujian Province, P. R. China, 350002

Core Summary

 The Plant Functional Chemical Genomics Core Facility provides high-throughput screening services to universities and research institutions nationwide. Currently, the facility houses a chemical library of plant natural compounds and a Tecan Freedom EVO® 200 multi-functional robotic workstation, a Spark® multifunctional microplate reader, a Cytomat™ automatic incubator for high throughput drug screening, enzyme-linked immunosorbent assays, and the establishment of cell-based drug screening models, etc. The facility is also equipped with a cleanroom suite and key instruments necessary for routine cell culture, and provide training and technical assistance for the growing demand of innovative scientific research.

List of Instruments

Tecan Freedom EVO® 200 workstation

 The Tecan Freedom EVO^® workstation is a flexible robotic platform for high throughput applications. The robot performs fully automated liquid-handling tasks with two pipetting arms and a manipulator arm. The workstation is integrated with the following peripheral devices:

• Te-Shake orbital shaker: This highly effective shaker could handle microplates, deep-well plates, half-skirted PCR plates, and microcentrifuge tubes. The shaker offers full software control of shaking parameters satisfying the needs of both rigorous mixing and delicate resuspension.

• MIO monitored incubation option: This unit provides temperature-controlled incubation directly on the workdeck.

• Hydrospeed Plate washer: This microplate washer precisely controls the speed and power of aspiration and dispensing for cell, bead and ELISA washing applications.

• Spark^ microplate reader: This spectrophotometer is configured to support multiple detection modes including absorbance, fluorescence (top & bottom), time resolved fluorescence, fluorescence polarization, and luminescence.

• Cytomat 2C incubator: This CO₂ plate incubator accommodates all standard microplate formats under various temperatures, humidity ranges, and gaseous atmospheres.

• Laminar flow cabinet: HEPA Class 100 laminar air flow cabinet maintains a constant positive air pressure to prevent the intrusion of contamination.

Mammalian Cell Culture Equipment

• Class 100,000 cleanroom suite

• Thermofisher ClassII Type A2 biological safety cabinet

• Thermofisher 4100 water-jacketed CO₂ incubator

• Thermofisher STERI-CYCLE i250 oxygen control (tri-gas) incubator

• Millipore Scepter 2.0 handheld automated cell counter

• Nikon Ts2-FL inverted fluorescence microscope

• TokaiHit TPi-TS2X ThermoPlate

• Thermofisher Locator JR liquid nitrogen tank

• balance

• centrifuge

• constant temperature shaking water bath